ICS65.020.20 CCSB05DB4419 东莞市地方标准 DB4419/T15—2024 朱顶红种苗组培快繁技术规程 TechnicalregulationsforproductionoftissuecultureplantletsofHippeastrum 2024–10–10发布 2024–11–01实施 东莞市市场监督管理局 发布DB4419/T15—2024 I目  次 前  言...............................................................................................................................................................III 1范围...................................................................................................................................................................1 2规范性引用文件..............................................................................................................................................1 3术语与定义......................................................................................................................................................1 4培养基..............................................................................................................................................................3 4.1初代培养基...............................................................................................................................................3 4.2增殖培养基...............................................................................................................................................3 4.3愈伤组织分化培养基...............................................................................................................................3 4.4体细胞胚成苗培养基...............................................................................................................................3 4.5壮苗培养基...............................................................................................................................................3 4.6生根培养基...............................................................................................................................................3 5组培苗快繁流程..............................................................................................................................................3 5.1培养基配制...............................................................................................................................................3 5.2培养基灭菌...............................................................................................................................................3 5.3接种工具灭菌...........................................................................................................................................3 5.4环境消毒...................................................................................................................................................4 5.5无菌操作...................................................................................................................................................4 5.6外植体选择...............................................................................................................................................4 5.7外植体消毒...............................................................................................................................................4 5.8初代培养...................................................................................................................................................4 5.8.1以鳞茎盘为外植体诱导不定芽.......................................................................................................4 5.8.2以鳞片为外植体诱导胚性愈伤组织...............................................................................................4 5.8.3以子房和花梗为外植体诱导不定芽...............................................................................................4 5.9增殖培养...................................................................................................................................................4 5.9.1不定芽增殖培养................................................................................................................................4 5.9.2愈伤组织增殖培养...................................................