DB61
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DB61/T 410-2007
2007-09-03~~ 2007-09-03~~
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~~m~mf••ff~~•••• *.~~o
, 2 ~~1115Im3t1tj:
-fjitl)(fi: ~00 *-.-~:ilI1:i1 **5]\mtr'.J 51 flHffi J£)!l **5]\moo t.k~ c }L~ff 8 W:I i¥J 51 m)({IF, ltmIJfiM1.:ri¥J
f~t:&~ (::f'§tliWJ~~ 00 P;J tP !IX1~iTAOC~::ffjm T **5]\m, rt[ffi, ~)}jjJ~~**JRft.i6J£t;1) ixEfJ ~nEff1\:
~1iiJf 1~J:l3:i! @)(1!I:ooliU~TAOC*o }L~::fff8 Jt)j i¥J 51 m)(1*, JtliiTAOC*Jfiffi T *;f~m 0
IFU 76 (2000 : iF1~jff~D-iFfi11 ••i¥J~g~
ATP 5'-=~M~1r
ADP 5'-=~"J1~1J
6-PGDH. 6-~MtJ••lmttll
NADP+ 1:Ii;mU~J1~p~nt -~tr~~~
NADPH J±Jjjm~:mJM;IJg(Wolnt =f.~1l~~.
U ji¥~1tt:EI ~'F~{i/:, 1'£ 25'C i¥Jf;J~mt.kf!l: -F1fJ: minIU i¥J.¥i5#J~$tt1tJ£ 1 Jl mol
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tE.4t1••~.i¥JfHR-f-, D-.4t1.~flT~H&5'-'::::~.J1~tt (ATP) ~.1t;JD-6-~~.1ii1 •••U:
;f115'---.:.~.JlI'Hr (ADP) JJl~A;J:0
illi'titj,Hl31~MD-lii••• + ATP .. D-6-~M.~Hm. + ADP
t£ 6-~~riiij ••)]£~Uii (6-PGDH) i¥JftF8 "I", D-6-~.ij ••mHBz:mJf:liU~H~n~1l7 -;f~1J.~.
(NADP) ~1t)]£tt)!l 5-~.t~.WIiij:fllJ±Jw)\'HtlM;H1i:JlI'~n~Il7-~~11.~. (NADPH)o 1Jt~A7g:
6-PGDH
D-6-~~ •••• + NADP+ .. 5-~.~.~ + NADPH + CO, + H"
~t.tEZ.f\Y-~ NADPH Jf5J£1¥J,8, 3 T D-ij••~,8,1$t. :ilI1:i1itEZ.Jlljjf5(1'± 340nm ~tl¥JP&7t7tltm
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, 5.1 NADP: -15'C~ -20'C~1f
l 5.2 ATP: -15'C~ -2o'CHf1:1f
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5.3 MgSO,' 7H10
DB61ff 410-2007
5.4 6-PGDH
5.5 1i~••iUfU~;¥~~
5.6 .=.ZM:1ni:
5. 7 Hel
5.8 NaOH
5.9 KOH
5. 10 :i:~*
5. 11 tB ~1.rem ]tlj,fb t~*Uif
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6. 1 l$1flm (5 mL, 1 mL 50-200 ilL, 10-50 II L)
6. 2 lt~JIll; 1[;:& L Ocm
6. 3 ~9r7t1tYtllir. ~~~*, 340nm ~U!E
6. 4 !1T!t~tJ£, fL1£ 10 II m
6. 5 ~,c.·m
6. 6 ~.c.\~, 1IEtf, ~:lJm, ~~:ffi:!G~~!k, ii4, ~J$J*,~
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7.1.1 =ZD~~/$Hi:
NADP (g~60mg) , ATP (~~150mg), Imol/L MgSO.· 7H 20 4ml, ~-r:27mL:~~7k, ~pH~7.6o
7. 1. 2 6-PGDH I i1=~i
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7.1. 3 1i1i••1J!(.~~Ri
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7.1.4 He]: 2 moIlL
7.1.5 NaOH: 2mollL.
7. 1. 6 KOH: mollL
7. 2 lit~*J*
7.2.1 jff:it:, 7C,\!, q::i't1fii*~~
X1m~, x13, tp'I"i~1*~,fb, ~1[~:itt1TtftWlIJ, 1tffll. OOO~l. 800mUfH&:itt1Ttft~~.
7.2. 2 ~i!I!~~
i3l:~~jEUf,fb, fflNaOH~nt~,~KOHm~~~~tfJpH~8ftti, 1tffl L OOO~l. 800mLm1fl:itt1TtftWltl 0
7.2. 3 *~1**jt
llt;~ff£tptfJCOl;:1*, fflNaOHm~~KOHm~~pH~8ftti, tf§Pnfjd~15min, 1tffll. OOO~1. 800mL
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7. 3 ~~J 01i~ 1 ilIff)
7.3.1 li£:S;i;j'lffi
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DB61-T 410-2007 葡萄汁中D-葡萄糖酸的测定—酶法 陕西省
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